anti mapk phosphatase 1 mkp 1 monoclonal antibody (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
94
Structured Review
Santa Cruz Biotechnology
anti mapk phosphatase 1 mkp 1 monoclonal antibody
Anti Mapk Phosphatase 1 Mkp 1 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mapk+phosphatase+1+mkp+1/MKP-1+Antibody/pmc11546882-105-0-16
Average 94 stars, based on 519 article reviews
Anti Mapk Phosphatase 1 Mkp 1 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mapk+phosphatase+1+mkp+1/MKP-1+Antibody/pmc11546882-105-0-16
Average 94 stars, based on 519 article reviews
anti mapk phosphatase 1 mkp 1 monoclonal antibody - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Co-Stimulation with TWEAK and TGF-β1 Induces Steroid-Insensitive TSLP and CCL5 Production in BEAS-2B Human Bronchial Epithelial Cells"
Article Title: Co-Stimulation with TWEAK and TGF-β1 Induces Steroid-Insensitive TSLP and CCL5 Production in BEAS-2B Human Bronchial Epithelial Cells
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms252111625
Figure Legend Snippet: Molecular mechanisms resulting in steroid unresponsiveness, induced by co-stimulation of TWEAK and TGF-β1. The mRNA levels of MKP-1 ( A ), GILZ ( C ), GRβ ( E ), and HDAC2 ( F ) mRNA after 48 h treatment, analyzed by qRT–PCR. MAP kinase phosphatase 1 (MKP-1) and glucocorticoid-induced leucine zipper (GILZ) levels analyzed by immunoblotting ( B , D , upper panel). Densitometric analysis of protein bands ( B , D , lower). Data represent mean ± SD of two independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX.
Techniques Used: Quantitative RT-PCR, Western Blot
Figure Legend Snippet: Co-stimulation of TWEAK and TGF-β1 induces steroid-unresponsive production of cytokines through the mitogen-activated protein kinase and nuclear factor kappa beta signaling pathways. Confluent monolayers of BEAS-2B cells were cultured for 48 h in the absence (DMSO as vehicle) or presence of AZD6244 (5 μM), SB202190 (5 μM), SP600125 (5 μM), or BAY11-7082 (2.5 μM) and treated with TGF-β1 (10 ng/mL), TWEAK (100 ng/mL), or TGF-β1 in combination with TWEAK. The mRNA levels of TSLP ( A ), CCL5 ( B ), MKP-1 ( C ), and GILZ ( D ) were analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. * p < 0.05, compared with the vehicle; † p < 0.05 compared with cultures in the absence of DEX.
Techniques Used: Protein-Protein interactions, Cell Culture, Quantitative RT-PCR
Figure Legend Snippet: MKP-1 is involved in steroid unresponsiveness induced by co-stimulation with TWEAK and TGF-β1 in BEAS-2B cells. The mRNA levels of MKP-1 ( A ), TSLP ( C ), and CCL5 ( E ), analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. MKP-1 and thymic stromal lymphopoietin (TSLP) expression after 48 h of treatment, assessed by immunoblotting ( B , D , upper panel). Densitometric analysis of protein bands ( B , D , lower). The level of CCL5 in cell culture supernatants after 48 h of treatment, analyzed using ELISA ( F ). Data represent mean ± SD of two independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX; ‡ p < 0.05 compared with control siRNA.
Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Control
Related Articles
other:Article Title: Hydroquinone regulates hemeoxygenase-1 expression via modulation of Src kinase activity through thiolation of cysteine residues. Article Snippet: The hydroxylated benzene metabolite hydroquinone (HQ) is mainly generated from benzene, an important industrial chemical, and is also a common dietary component.. Although numerous papers have addressed the potential role of HQ in tumorigenic responses, the immunosuppressive and antiinflammatory effects of hydroquinone have also been considered.. In this study, we characterized the mechanism of the induction of hemeoxygenase (HO)-1 and other phase 2 enzymes by HQ and its derivatives. Article Title: Prolongation of insulin-induced activation of mitogen-activated protein kinases ERK 1/2 and phosphatidylinositol 3-kinase by vanadyl sulfate, a protein tyrosine phosphatase inhibitor. Article Snippet: Vanadium salts such as vanadyl sulfate (VS), potent inhibitors of protein tyrosine phosphatases, have been shown to mimic, augment, and prolong insulin s action.. However, the molecular mechanism of responses to these salts is not clear.. In the present studies, we examined if VS-induced effects on insulin action are associated with enhancement or augmentation in the activation state of key components of the insulin signaling pathway. Incubation:Article Title: Proteasome inhibition reduces proliferation, collagen expression, and inflammatory cytokine production in nasal mucosa and polyp fibroblasts. Article Snippet: .. Membranes were preincubated for 1 h at room temperature in 10 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 0.1% Tween 20 containing either 5% fat-free powdered milk or 5% BSA, and then incubated (overnight at 4°C) with the primary antibodies against p27 (Santa at A SPE T Journals on M arch 6, 2015 jpet.aspetjournals.org D ow nloaded from Cruz Biotechnology, Inc., Santa Cruz, CA), total retinoblastoma (Rb; Santa Cruz Biotechnology, Inc.), cyclin D1 (Santa Cruz Biotechnology, Inc.), total c-Jun (Santa Cruz Biotechnology, Inc.), and phosphorylated c-Jun (Ser63; Santa Cruz Biotechnology, Inc.), Plasmid Preparation:Article Title: pp90RSK- and protein kinase C-dependent pathway regulates p42/44MAPK-induced LDL receptor transcription in HepG2 cells Article Snippet: Phospho-specific antibodies to the activated forms of p42/ 44 MAPK (Thr202/Tyr204), and phospho-pp90 RSK (Ser259) were purchased from New England Biolabs. .. Antibodies to Raf-1 kinase, p42/44 MAPK and Binding Assay:Article Title: pp90RSK- and protein kinase C-dependent pathway regulates p42/44MAPK-induced LDL receptor transcription in HepG2 cells Article Snippet: Phospho-specific antibodies to the activated forms of p42/ 44 MAPK (Thr202/Tyr204), and phospho-pp90 RSK (Ser259) were purchased from New England Biolabs. .. Antibodies to Raf-1 kinase, p42/44 MAPK and |